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カワムラ クニオ
KAWAMURA Kunio 川村 邦男 所属 広島修道大学 人間環境学部 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2022/08 |
| 形態種別 | 学術論文 |
| 査読 | 査読有り |
| 標題 | A high-pressure, high-temperature flow reactor simulating the hadean earth environment, with application to the pressure dependence of the cleavage of avocado viroid hammerhead ribozyme |
| 執筆形態 | 共著 |
| 掲載誌名 | Life |
| 掲載区分 | 国外 |
| 出版社・発行元 | MDPI |
| 巻・号・頁 | 12,pp.1224 |
| 頁数 | 13 |
| 担当範囲 | Corresponding author |
| 著者・共著者 | Kunio Kawamura, Mari Ogawa, Noriko Konagaya, Yoshimi Maruoka, Jean-François Lambert, Louis M. P. Ter-Ovanessian, Jacques Vergne, Guy Hervé, Marie-Christine Maurel |
| 概要 | The RNA world hypothesis suggests that chemical networks consisting of functional RNA molecules could have constructed a primitive life-like system leading a first living system. We have demonstrated the importance of the environment at both high temperature and high pressure, using different types of hydrothermal flow reactor systems and high-pressure equipment. In the present study, we have attempted to develop an alternative easy-to-implement method for high-pressure mea- surements and demonstrate that the system is applicable as an efficient research tool for high-pressure experiments at pressures up to 30 MPa. We demonstrate the usefulness of the system by detecting the high-pressure influence for the self-cleavage of avocado hammerhead ribozyme (ASBVd(−):HHR) at 45–65 ◦C. A kinetic analysis of the high-pressure behavior of ASBVd(−):HHR shows that the ribozyme is active at 30 MPa and its activity is sensitive to pressures between 0.1–30 MPa. |