カワムラ　クニオ   KAWAMURA Kunio   川村　邦男    所属   広島修道大学  人間環境学部    職種   教授
言語種別	英語
発行・発表の年月	2024/01
形態種別	学術論文
査読	査読有り
標題	HPLC retention behavior of oligonucleotides on an anion-exchange HPLC with a high concentration of urea
執筆形態	共著
掲載誌名	Chromatographia
掲載区分	国外
出版社・発行元	Springer
巻・号・頁	87,pp.17-25
頁数	9
担当範囲	All
著者・共著者	◎Kunio Kawamura, Yoshimi Maruoka
概要	In this study, the HPLC separation behavior in the presence of a high urea concentration is studied using a Tosoh DNA-NPR anion-exchange column with a buffer containing 0.02 M Tris and 7.5 M urea at pH 9.0 and a buffer containing 1.5 M NaCl, 0.02 M Tris, and 7.5 M urea at pH 9.0 mixed with a linear gradient at a flow rate of 0.15 mL min−1 at 35 °C. Different sequences of oligonucleotides are collected to know how the influence of secondary structure formation can be reduced. Molecular modeling of the secondary structure is applied to evaluate the HPLC retention behaviors of oligonucleotides on an anion-exchange column. This study provides valuable information for selecting the elution conditions for separating the RNA and DNA oligonucleotides and preheating treatment.