カワムラ　クニオ   KAWAMURA Kunio   川村　邦男    所属   広島修道大学  人間環境学部    職種   教授
言語種別	英語
発行・発表の年月	2001/12
形態種別	学術論文
査読	査読有り
標題	Cyclization and dimerization of hexanucleotides containing guanine and cytosine with water-soluble carbodiimide
執筆形態	共著
掲載誌名	Viva Origino
掲載区分	国内
巻・号・頁	29(4),pp.162-167
著者・共著者	◎Kunio Kawamura, Fumitaka Okamoto
概要	The condensation reaction of hexanucleotides 5'-pGGGCCrC, 5'-pGCGCGrC, and 5'-pGCCCGrG was investigated in the presence of water-soluble carbodiimide (WSC) and imidazole at 0 oC. The efficiency of the condensation of hexanucleotides with WSC was investigated to know whether these hexanucleotides act as a partial template to promote the elongation reaction. The condensation reaction mainly yielded cyclic- hexanucleotides (30 - 60 %) and small amount of 12-mers (< 10 %), which opposed our expectation. The yield of cyclization was in the order 5'-pGGGCCrC ≅ 5'-pGCGCGrC > 5'-pGCCCGrG and that of dimerization was in the order 5'-pGGGCCrC ≅ 5'-pGCGCGrC < 5'-pGCCCGrG. The trend to cause efficient cyclization could be an obstacle at an early stage of chemical evolution of RNA.