カワムラ　クニオ   KAWAMURA Kunio   川村　邦男    所属   広島修道大学  人間環境学部    職種   教授
言語種別	英語
発行・発表の年月	2024/12
形態種別	学術論文
査読	査読有り
標題	Linear oligopeptide formation from alanine-diketopiperazine in acidic aqueous solutions using interfacial nano-pulsed discharge plasma
執筆形態	共著
掲載誌名	New Journal of Chemistry
掲載区分	国外
出版社・発行元	Royal Society of Chemistry
巻・号・頁	49,pp.514-520
頁数	7
担当範囲	全体　Corresponding　author
著者・共著者	Mitsuru Sasaki, Kouki Nonaka, Yuka Sakai, Tetsuo Honma, Tomohiro Furusato, Kunio Kawamura,
概要	Traditionally, synthesis of peptides using solid- or liquid-phase methods requires organic solvents, which goes against the fundamentals of green chemistry. By optimizing the conditions for oligopeptide synthesis, we improved the green synthesis of alanine oligopeptides by performing plasma irradiation in an acidic aqueous solution. The yield of alanyl–alanyl–alanine from alanine–DKP is approximately 30% within 3–20 min. The addition of alanylalanine enhances the formation of higher oligopeptides. Furthermore, LC-MS analysis shows trace amounts of glycine–DKP, glycine oligopeptides, glutamic acid, pyruvic acid, and pyroglutamic acid, which implies the reaction mechanism for the spontaneous elongation of higher oligopeptides from alanine–DKP through the ring opening of alanine–DKP and the radical for- mation of amino acids. Thus, the formation of DKP is advantageous for the formation of oligopeptides and does not inhibit oligopeptide elongation.