カワムラ　クニオ   KAWAMURA Kunio   川村　邦男    所属   広島修道大学  人間環境学部    職種   教授
言語種別	英語
発行・発表の年月	1994/08
形態種別	学術論文
査読	査読有り
標題	Kinetics and mechanistic analysis of dinucleotide and oligonucleotide formation from the 5'-phosphorimidazolide of adenosine on Na+-montmorillonite
執筆形態	共著
掲載誌名	J. Am. Chem. Soc.
掲載区分	国外
巻・号・頁	116(17),pp.7564-7572
著者・共著者	◎Kunio Kawamura, James P. Ferris
概要	The rate constants for the condensation reaction of the 5'-phosphorimidazolide of adenosine (ImpA) to form dinucleotides and oligonucleotides havebeen measuredin the presence of Na+-volclay (a Na+-montmorillonite) in pH 8 aqueous solution at 25 C. The binding of ImpA to montmorillonite was measured, and the adsorption isotherm was determined. The binding of ImpA to montmorillonite and the formationof higheroligonucleotides is not observed in the absence of salts. Mg2+ enhances binding and oligonucleotide formation more than Ca2+ and Na+. The rate constants for the oligonucleotide formation were determined from the reaction products formed from 10 to 40 mM ImpA in the presence of Na+-montmorillonite using the computer program SIMFIT. The magnitudes of the rate constants for the formation of oligonucleotides increased in the order 2-mer< 3-mer< 4-mer··· 7-mer.