カワムラ　クニオ   KAWAMURA Kunio   川村　邦男    所属   広島修道大学  人間環境学部    職種   教授
言語種別	英語
発行・発表の年月	1996/04
形態種別	学術論文
査読	査読有り
標題	Oligomerization of uridine phosphorimidazolides on montmorillonite: A model for the prebiotic synthesis of RNA on minerals
執筆形態	共著
掲載誌名	Origins Life Evol. Biosphere
掲載区分	国外
巻・号・頁	26(2),pp.151-171
著者・共著者	◎Ping Z. Ding, Kunio Kawamura, James P. Ferris
概要	The 5′-phosphorimidazolide of uridine reacts on Na+-montmorillonite 22A in aqueous solution to give oligomers as long as 7 mers. The maximum chain length increases to 9 mers and the overall oligomer yield increases when 9:1 ImpU, A5′ ppA mixtures react under the same conditions. The oligomer yield and maximum chain length decreases with the structure of the added pyrophosphate in the order A5′ ppA>A5′ ppU>U5′ ppU. Structure analysis of individual oligomer fractions was performed by selective enzymatic hydrolyses followed by HPLC analysis of the products. The regioselectivity for 3′,5′-bond formation is 80–90% in the 9:1 ImpU, A5′ ppA reaction, a percentage comparable to that observed in the 9:1 ImpA, A5′ ppA reaction. Oligomerization of ImpU is inhibited by addition of dA5′ ppdA, and MeppA.