カワムラ　クニオ   KAWAMURA Kunio   川村　邦男    所属   広島修道大学  人間環境学部    職種   教授
言語種別	英語
発行・発表の年月	2003/12
形態種別	学術論文
査読	査読有り
標題	Temperature dependence of the cyclization of guanine and cytosine mix hexanucleotides with water-soluble carbodiimide at 0 -75 oC
執筆形態	共著
掲載誌名	Viva Origino
掲載区分	国内
巻・号・頁	31(4),pp.221-232
担当範囲	Corresponding author
著者・共著者	◎Kunio Kawamura, Noriyuki Nakahara, Fumitaka Okamoto, Noriko Okuda
概要	The temperature dependence of pseudo-first-order rate constants (kcyc) of the cyclization of hexanucleotides 5'-d(pGCGCG)rC and 5'- d(pGCCCG)rG was investigated in the presence of 1- ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC) and imidazole at 0 - 75 °C. Although the association between the elongating oligomer and the activated monomer is necessary for the success of the formation of oligonucleotides on a polynucleotide template or a clay catalyst, the phosphodiester bond formation in the case of cyclization readily occurs without these catalysts. Thus, the cyclization is regarded as a simple model of the formation of phosphodiester bond. The rate constants (kcyc) were compared with those of the cleavage of the ribose phosphodiester bond, in which the cyclization was more than 50 times faster than the cleavage at 75 °C.