ニッタ　ユミコ   NITTA Yumiko   新田　由美子    所属   広島修道大学  健康科学部    職種   教授
言語種別	英語
発行・発表の年月	2002/03
形態種別	学術論文
査読	査読有り
標題	The quality of DNA recovered from the archival tissues of Atomic bomb survivors is good enough for the single nucleotide polymorphism analysis in spite of the decade-long preservation in formalin.
執筆形態	共著
掲載誌名	J. Radiat. Res.,
掲載区分	国内
巻・号・頁	43,pp.65-75
頁数	11
著者・共著者	◎Nitta Y., Tanaka H., Masuda Y., Hoshi M.
概要	“Attempts to Search for SNPs Using DNA Extracted from Long-Term Formalin-Fixed Organs of Atomic Bomb Survivors” Autopsy organs of atomic bomb survivors are typically preserved long-term in formalin. Examination of formalin's effects on the genome revealed reduced DNA recovery rates. This is due to the aldehyde groups in formalin causing fragmentation of the bases constituting DNA. We clarified that this phenomenon exhibits temperature dependence, time dependence, and pH dependence. We demonstrated that DNA amplification of approximately 50 base pairs is possible even from these fragmented short genomic fragments.
researchmap用URL	https://academic.oup.com/jrr/article/43/1/65/910226?login=true