カワムラ　クニオ   KAWAMURA Kunio   川村　邦男    所属   広島修道大学  人間環境学部    職種   教授
言語種別	英語
発行・発表の年月	2004/07
形態種別	学術論文
査読	査読有り
標題	Prebiotic inhibitory activity of protein-like molecules to the template-directed formation of oligoguanylate from guanosine 5’-monophosphate 2-methylimidazolide on a polycytidylic acid template
執筆形態	共著
掲載誌名	Bull. Chem. Soc. Jpn.
掲載区分	国外
巻・号・頁	77(7),pp.1367-1375
担当範囲	Corresponding author
著者・共著者	◎Kunio Kawamura, Kazuhiro Kuranoue, Minoru Nagahama
概要	The influence of thermal copolymers of amino acids (TCAA) as a primitive protein enzyme model has been inves- tigated to detect catalytic and/or inhibitory activities on the template-directed formation of oligoguanylate (oligo(G)) from guanosine 50-monophosphate 2-methylimidazolide (2-MeImpG) on a polycytidylic acid template (poly(C)) (TD re- action). Different amino acids and compositions were used to prepare TCAAs. These TCAAs were characterized by gel filtration HPLC and the composition analysis of amino acids using phenyl isothiocyanate. It was found that TCAA includ- ing His (TCAA-His) has inhibitory activity to the TD reaction, in which TCAA-His accelerates the hydrolysis of 2- MeImpG. Other types of TCAAs, including Gly, Ala, Val, Glu, and Asp, also accelerate the formation of P1,P2-bis(50- guanosyl) diphosphate (G50 ppG) and G50 ppG incorporated oligo(G)s during the TD reaction at high concentrations of TCAAs.