カワムラ　クニオ   KAWAMURA Kunio   川村　邦男    所属   広島修道大学  人間環境学部    職種   教授
言語種別	英語
発行・発表の年月	2005/09
形態種別	学術論文
査読	査読有り
標題	A new probe for the indirect measurement of the conformation and interaction of biopolymers at extremely high temperatures using a capillary flow hydrothermal reactor system for UV-visible spectrophotometry
執筆形態	単著
掲載誌名	Anal. Chim. Acta
掲載区分	国外
巻・号・頁	543(1-2),pp.236-241
概要	The scope of the capillary flow hydrothermal reactor system for the UV–vis spectrophotometric detection (CHUS), which was recently developed in our group for the in situ UV–vis monitoring of hydrothermal reactions at the millisecond–second time range, has been investigated as a potential new probe for the analysis of conformations and interactions of polynucleotides at extremely high temperatures. It was possible to detect indirectly the difference between double-stranded and single-stranded DNA on the basis of the intercalation with ethidium bromide (EB). Measurements of absorbance of the DNA–EB complex were attempted at up to 200◦C by the CHUS system, and the results were consistent with the melting behavior observed by differential scanning calorimetry. This study supports that the CHUS method is applicable as a new probe for exploring interactions and conformation within polynucleotides at high temperatures, such as in hyperthermophilus.