カワムラ　クニオ   KAWAMURA Kunio   川村　邦男    所属   広島修道大学  人間環境学部    職種   教授
言語種別	英語
発行・発表の年月	2000/11
形態種別	著書
招待	招待あり
標題	"Capillary Electrophoresis of Nucleic Acids" in "Encyclopedia of Analytical Chemistry"
執筆形態	共著
掲載区分	国外
出版社・発行元	John Wiley&Sons Ltd
巻・号・頁	6,pp.4871-4893
担当範囲	Capillary Electrophoresis of Nucleic Acids
著者・共著者	Kunio Kawamura
概要	Capillary electrophoresis (CE) is a powerful and rela- tively new technique for nucleic acid mapping, and can be used along with conventional slab electrophoresis and high-performance liquid chromatography (HPLC). There are several types of separation techniques of CE all of which are capable of being used for quantification and qualification of nucleic acids. CGE and CAGE, in which electrophoresis is performed in molecular sieving materials of polymer gels, are used rather than HPLC and slab gel electrophoresis (SGE) for sequencing of large nucleic acids for several reasons: high-resolution power, high-speed sequencing, ease of automation, and small sam- ple volume. Combining the advantages of CGE and laser fluorescence detection with a four- or two-color label- ing method enables sequencing of more than 1000 bases within 1h.