カワムラ　クニオ   KAWAMURA Kunio   川村　邦男    所属   広島修道大学  人間環境学部    職種   教授
言語種別	英語
発行・発表の年月	2007/12
形態種別	学術論文
査読	査読有り
標題	Behavior of DNA under hydrothermal conditions with MgCl2 additive using an in situ UV-visible spectrophotometer
執筆形態	共著
掲載誌名	Themchim. Acta
掲載区分	国外
巻・号・頁	466,pp.63-68
担当範囲	Corresponding author
著者・共著者	◎Kunio Kawamura, Hiroki Nagayoshi
概要	The capillary flow hydrothermal reactor system for the UV–vis spectrophotometric detection system (CHUS) has been applied to inspect the behavior of DNA at temperatures up to 300◦C. Double-stranded DNA (dsDNA) was monitored with and without ethidium bromide in the presence of MgCl2 . The ratio (A+DNA /A−DNA ) of the absorbance of EB with DNA (A+DNA ) to that without DNA (A−DNA ) increased steeply over 1 at temperatures around 100◦C, which was not observed in the absence of MgCl2. This was found to be due to the decrease of solubility of DNA in hot water, where dsDNA is converted to single-stranded DNA and become insoluble. At temperatures over 175◦C, DNA becomes soluble again because of the degradation of long insoluble DNA polymers to short soluble DNA oligomers. This study points out the importance of solubility of DNA for life at extremely high temperatures as well as the stability of DNA.