カワムラ　クニオ   KAWAMURA Kunio   川村　邦男    所属   広島修道大学  人間環境学部    職種   教授
言語種別	英語
発行・発表の年月	2009/02
形態種別	学術論文
査読	査読有り
招待	招待あり
標題	Stability of ribonuclease A under hydrothermal conditions in relation to the origin-of-life hypothesis: Verification with the hydrothermal micro-flow reactor system
執筆形態	共著
掲載誌名	Res. Chem. Intermed.
掲載区分	国外
巻・号・頁	35(8-9),pp.879-891
担当範囲	Corresponding author
著者・共著者	◎Kunio Kawamura, Hiroki Nagayoshi, Toshio Yao
概要	The stability of ribonuclease A (RNase A) was quantitatively investigated with the hydrothermal micro-flow reactor system (HFRS) at tem- peratures of up to 275 °C from the viewpoint of the hydrothermal origin-of-life hypothesis. The enzymatic activity of RNase A was studied with regard to the catalytic degradation of polynucleotides with anion-exchange high-performance liquid chromatography, while the degradation of RNase A to shorter molecules was analyzed by size exclusion chromatography (SEC) and mass spectrometry (MS). The degradation of RNase A started within 10 s at 200 °C, and the enzymatic activity disappeared almost completely after 25 s. SEC and MS analyses indicated that RNase A was thermally degraded to 2 large fragments, which, along with RNase A, were further decomposed to smaller fragments. This study showed that RNase A is fairly stable under normal conditions, but its enzymatic activity disappears rapidly at extremely high temperatures.