カワムラ　クニオ   KAWAMURA Kunio   川村　邦男    所属   広島修道大学  人間環境学部    職種   教授
言語種別	英語
発行・発表の年月	2010/05
形態種別	学術論文
査読	査読有り
標題	Facile preparation method of a disposable capillary biosensor using an ion-selective optode membrane and a dissolvable enzyme membrane and its application to urea sensing
執筆形態	共著
掲載誌名	Chem. Lett.
掲載区分	国内
巻・号・頁	39(5),pp.436-438
著者・共著者	◎Erina Tsutsumi, Terence G. Henares, Kunio Kawamura, Toshio Yao, Hideaki Hisamoto
概要	A facile preparation method of a disposable capillary biosensor immobilizing a plasticized poly(vinyl chloride) (PVC) membrane ion-selective optode containing a lipophilic pH indicator dye and an ionophore, and a dissolvable poly- (ethylene glycol) (PEG) membrane containing an enzyme is developed. The method involves the sequential introduction of membrane cocktail solution followed by air introduction. Repeating this process allowed facile preparation of a capillary biosensor comprised of two layer structure, which can be used for single-step biosensing just by sipping the sample with capillary action. Here a urea biosensor was prepared as a proof-of-concept.