カワムラ　クニオ   KAWAMURA Kunio   川村　邦男    所属   広島修道大学  人間環境学部    職種   教授
言語種別	英語
発行・発表の年月	2010/05
形態種別	学術論文
査読	査読有り
標題	In situ analysis of proteins at high temperatures mediated by capillary-flow hydrothermal UV-Vis spectrophotometer with a water-soluble chromogenic reagent
執筆形態	共著
掲載誌名	Anal. Chim. Acta
掲載区分	国外
巻・号・頁	667,pp.88-95
担当範囲	Corresponding author
著者・共著者	◎Kunio Kawamura, Hiroki Nagayoshi, Toshio Yao
概要	In situ monitoring of quantities, interactions, and conformations of proteins is essential for the study of biochemistry under hydrothermal environments and the analysis of hyperthermophilic organisms in natural hydrothermal systems on Earth. We have investigated the potential of a capillary-flow hydrothermal UV–vis spectrophotometer (CHUS) for performing in situ measurements of proteins and determining their behavior at extremely high temperatures, in combination with a chromogenic reagents probe, which interacts with the proteins. The association behavior of TPPS with BSA was investigated in detail using CHUS at temperatures up to 175 ◦ C and the association constant (Kass ) of TPPS with BSA was successfully determined at temperatures up to 100 ◦ C.