カワムラ　クニオ   KAWAMURA Kunio   川村　邦男    所属   広島修道大学  人間環境学部    職種   教授
言語種別	英語
発行・発表の年月	2010/07
形態種別	学術論文
査読	査読有り
標題	Single-step ELISA capillary sensor based on surface-bonded glucose oxidase, antibody, and physically-adsorbed PEG membrane containing peroxidase-labeled antibody
執筆形態	共著
掲載誌名	Sens. Actuators B.
掲載区分	国外
巻・号・頁	149,pp.319-324
著者・共著者	◎Terence G. Henares, Erina Tsutsumi, Hiromi Yoshimura, Kunio Kawamura, Toshio Yao, Hideaki Hisamoto
概要	A single-step enzyme-linked immunosorbent assay (ELISA) capillary immunosensor that exploits the combination of surface-bonded glucose oxidase (GOD), antibody and physically-adsorbed poly(ethyleneglycol) (PEG) membrane containing peroxidase (POD)-labeled antibody in a single cap- illary, is presented.Preliminary results yielded an approximate detection limit of around subnanogram per milliliter concentration for human IgG. The relative standard deviation of the fluorescence response was ca. 4.1% for the capillary pieces prepared by cutting a long immunosensor capillary. This approach could be an enabling tech- nology applicable to microfluidic device integration for multiple analyte sensing. Realization of such an endeavor could be very promising for drug screening and clinical diagnostic applications.