カワムラ　クニオ   KAWAMURA Kunio   川村　邦男    所属   広島修道大学  人間環境学部    職種   教授
言語種別	英語
発行・発表の年月	2010/08
形態種別	学術論文
査読	査読有り
標題	Reagent-release capillary array-isoelectric focusing device as a rapid screening device for IEF condition optimization
執筆形態	共著
掲載誌名	Lab. Chip.
掲載区分	国外
巻・号・頁	10,pp.3341-3347
著者・共著者	◎Masaki Kataoka, Hiroki Yokoyama, Terence G. Henares, Kunio Kawamura, Toshio Yao, Hideaki Hisamoto
概要	This report describes the fabrication and characterization of a simple and disposable capillary isoelectric focusing (cIEF) device containing a reagent-release capillary (RRC) array and poly(dimethylsiloxane) (PDMS) platform, which allows rapid (within 10 min) screening of cIEF conditions by introducing a sample solution into plural RRCs by capillary action followed by electric field application. When the sample solution containing proteins was introduced into the RRC, physically adsorbed CA, surfactant, and LR can be dissolved and released into the sample solution. Then, complexation of LR with proteins, mixing with CA and surfactant, and exposure of the PDMA surface spontaneously occurs for the IEF experiments. Here, three different RRCs that immobilize different CAs were prepared, and simultaneous cIEF experiments involving hemoglobin AFSC mixtures for choosing the best CA demonstrated the proof of concept.