オカニシ　マサノリ   OKANISHI Masanori   岡西　政典    所属   広島修道大学  人間環境学部    職種   教授
言語種別	英語
形態種別	学術論文
査読	査読有り
標題	Environmental DNA metabarcoding of benthic brittle stars (Echinodermata: Ophiuroidea) from Ise Bay, central Japan, targeting the 16S rRNA genes
執筆形態	共著
掲載誌名	Plankton & Benthos Research
掲載区分	国外
巻・号・頁	21(2),pp.99-110
頁数	12
担当範囲	サンプルの解析と論文執筆
著者・共著者	◎Masanori Okanishi, Luna Yamamori, Hideaki Miyashita
概要	Environmental DNA (eDNA) metabarcoding is increasingly used to monitor marine biodiversity, but benthic organisms have rarely been targeted, especially in Japan. We collected 20 L of surface and bottom seawater from depths of 17 m and 34 m in Ise Bay, central Japan, and analyzed brittle star eDNA using the mitochondrial 16S rRNA primer set 16SOph1. Metabarcoding detected five brittle star species, whereas direct collection detected only two. DNA from bottom-dwelling species was also detected in surface water. These results indicate that brittle star metabarcoding is feasible at depths of several tens of meters and that benthic eDNA can be transported vertically to surface waters.