カワムラ　クニオ   KAWAMURA Kunio   川村　邦男    所属   広島修道大学  人間環境学部    職種   教授
言語種別	英語
発行・発表の年月	2012/01
形態種別	学術論文
査読	査読有り
標題	Combinable poly(dimethyl siloxane) capillary sensor array for single-step and multiple enzyme inhibitor assays
執筆形態	共著
掲載誌名	Lab on a Chip
掲載区分	国外
巻・号・頁	12(1),pp.204-208
著者・共著者	◎Yosuke Uchiyama, Fumito Okubo, Kozo Akai, Yuji Fujii, Terence G. Henares, Kunio Kawamura, Toshio Yao, Tatsuro Endo, Hideaki Hisamoto
概要	We describe a new method for fabricating a capillary-type sensor, called a combinable poly(dimethyl siloxane) (PDMS) capillary (CPC) sensor. The method for preparing the CPC simplifies enzyme inhibitor assays into a simple, single step assay. The sample inhibitor solution is introduced by capillary action. This method allows the immobilization of two reactive reagents, such as enzyme and substrate required for a single step assay, which are typically very difficult to immobilize using commercially available conventional capillaries. Furthermore, by simply arraying various CPCs, the CPC sensor allows multiple assays. Here, we carried out a single-step enzyme inhibitor assay using the CPC. In addition, two independent CPCs were arrayed to demonstrate multiple assaying of a protease inhibitor.