カワムラ　クニオ   KAWAMURA Kunio   川村　邦男    所属   広島修道大学  人間環境学部    職種   教授
言語種別	英語
発行・発表の年月	2012/01
形態種別	学術論文
査読	査読有り
標題	Single-step sandwich immunoreaction in a square glass capillary immobilizing capture and enzyme-linked antibodies for simplified enzyme-linked immunosorbent assay
執筆形態	共著
掲載誌名	Anal. Sci.
掲載区分	国外
巻・号・頁	28,pp.51-56
著者・共著者	◎Erina Tsutsumi, Terence G. Henares, Shun-ichi Funano, Kunio Kawamura, Tatsuro Endo, Hideaki Hisamoto
概要	To simplify the complicated operation steps and to minimize sample and reagent amounts for enzyme-linked immunosorbent assays (ELISA), we developed a square glass capillary immunosensor containing both covalently immobilized capture antibodies and physically adsorbed enzyme-linked antibodies. We obtained an intra-capillary precision with a coefficient of variation of 9.7%. In addition, the specificity study showed that the human IgG capillary immunosensor did not respond to rabbit IgG. Quantitative analysis was possible within the response range of 10 – 5000 ng mL–1 anti-human IgG. This capillary immunosensor can act as a single analytical unit or can be integrated into a capillary array for multiple bioanalysis.