カワムラ　クニオ   KAWAMURA Kunio   川村　邦男    所属   広島修道大学  人間環境学部    職種   教授
言語種別	英語
発行・発表の年月	2012/04
形態種別	学術論文
査読	査読有り
標題	Bulk- and surface-modified combinable PDMS capillary sensor array as easy-to-use sensing device with enhanced sensitivity to elevated concentrations of multiple serum sample components
執筆形態	共著
掲載誌名	Lab Chip
掲載区分	国外
巻・号・頁	12,pp.1522-1526
著者・共著者	◎Yuji Fujii, Terence G. Henares, Kunio Kawamura, Tatsuro Endo, Hideaki Hisamoto
概要	To enhance sensitivity and facilitate easy sample introduction into a combinable poly(dimethylsiloxane) (PDMS) capillary (CPC) sensor array, PDMS was modified in bulk and on its surface to prepare ‘‘black’’ PDMS coated with a silver layer and self-assembled monolayer (SAM). India ink, a traditional Japanese black ink, was added to the PDMS pre-polymer for bulk modification. The surface was modified by a silver mirror reaction followed by SAM formation using cysteine. These modifications enhanced the fluorescence signals by reflecting them from the surface and reducing background interference. When serum samples were introduced by capillary action, the CPC sensor array showed fluorescence responses for each analyte and successfully identified the components with elevated concentrations in the serum samples.