カワムラ　クニオ   KAWAMURA Kunio   川村　邦男    所属   広島修道大学  人間環境学部    職種   教授
言語種別	英語
発行・発表の年月	2012/09
形態種別	学術論文
査読	査読有り
招待	招待あり
標題	Behavior of a hammerhead ribozyme in aqueous solution at medium to high temperatures
執筆形態	共著
掲載誌名	Naturwissenschaften
掲載区分	国外
巻・号・頁	99,pp.731-738
頁数	8
担当範囲	Corresponding author
著者・共著者	◎Nizar El-Murr, Marie-Christine Maurel, Martina Rihova, Jacques Vergne, Guy Hervé, Mikio Kato, Kunio Kawamura (corresponding author)
概要	The “RNA world” hypothesis proposes that— early in the evolution of life—RNA molecules played important roles both in information storage and in enzymatic functions. In the present work, self-cleavage of a natural hammerhead ribozyme was examined at temperatures 10–200 °C. Self-cleavage was investigated in the presence of Mg2+, which facilitates and accelerates this reaction. Self-cleavage of the hammerhead ribozyme was clearly observed at temperatures up to 60 °C, but at higher temperatures self-cleavage occurs together with hydrolysis and with increasing temperature hydrolysis becomes dominant. In addition, we discovered that the reaction proceeds in the presence of high concentrations of monovalent cations (Na+ or K+), although very slowly. Furthermore, at high temperatures (above 60 °C), monovalent cations protect the ribozyme against degradation.