カワムラ　クニオ   KAWAMURA Kunio   川村　邦男    所属   広島修道大学  人間環境学部    職種   教授
言語種別	英語
発行・発表の年月	2015/03
形態種別	学術論文
査読	査読有り
標題	Separation Behavior of Short Oligonucleotides by Ion-Pair Reversed-Phase Capillary Liquid Chromatography Using a Silica-Based Monolithic Column Applied to Simple Detection of SNPs
執筆形態	共著
掲載誌名	Chromatographia
掲載区分	国外
巻・号・頁	78,pp.487-494
担当範囲	Corresponding author
著者・共著者	◎Kunio Kawamura, Keisuke Ikoma, Yoshimi Maruoka, Hideaki Hisamoto
概要	The separation behaviors of short oligonu- cleotides using capillary liquid chromatography have been investigated comparatively on an octadecylsilane (ODS) packed column and a monolithic ODS column. The present paper clearly dem- onstrates that the resolution obtained using the monolithic ODS packed column is much higher than that using a semi-μ-high-performance liquid chromatography (HPLC) column on a semi-μ-HPLC system. Furthermore, it was surprising that complete baseline resolution of oligonucleo- tides, typically shorter than 30-mer, with single nucleotide units was achieved using the monolithic column, although this was not possible using the ODS packed column. Good separation of short oligonucleotides was achieved at 40–60 °C with a linear 40–60 % gradient of methanol content. Using the present method, detection of single-nucleotide polymorphisms was achieved without using a mass-spectrometry (MS) detector.