Department   Hiroshima shudo University  The Faculty of Human Environmental Studies
   Position   Professor
Language English
Publication Date 2022/08
Type Articles
Peer Review With peer review
Title A high-pressure, high-temperature flow reactor simulating the hadean earth environment, with application to the pressure dependence of the cleavage of avocado viroid hammerhead ribozyme
Contribution Type Co-Authored Publication
Journal Life
Journal TypeAnother Country
Publisher MDPI
Volume, Issue, Pages 12,pp.1224
Number of pages 13
Responsible for Corresponding author
Author and coauthor Kunio Kawamura, Mari Ogawa, Noriko Konagaya, Yoshimi Maruoka, Jean-François Lambert, Louis M. P. Ter-Ovanessian, Jacques Vergne, Guy Hervé, Marie-Christine Maurel
Details The RNA world hypothesis suggests that chemical networks consisting of functional RNA molecules could have constructed a primitive life-like system leading a first living system. We have demonstrated the importance of the environment at both high temperature and high pressure, using different types of hydrothermal flow reactor systems and high-pressure equipment. In the present study, we have attempted to develop an alternative easy-to-implement method for high-pressure mea- surements and demonstrate that the system is applicable as an efficient research tool for high-pressure experiments at pressures up to 30 MPa. We demonstrate the usefulness of the system by detecting the high-pressure influence for the self-cleavage of avocado hammerhead ribozyme (ASBVd(−):HHR) at 45–65 ◦C. A kinetic analysis of the high-pressure behavior of ASBVd(−):HHR shows that the ribozyme is active at 30 MPa and its activity is sensitive to pressures between 0.1–30 MPa.