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KAWAMURA Kunio
Department Hiroshima shudo University The Faculty of Human Environmental Studies Position Professor |
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| Language | English |
| Publication Date | 2022/08 |
| Type | Articles |
| Peer Review | With peer review |
| Title | A high-pressure, high-temperature flow reactor simulating the hadean earth environment, with application to the pressure dependence of the cleavage of avocado viroid hammerhead ribozyme |
| Contribution Type | Co-Authored Publication |
| Journal | Life |
| Journal Type | Another Country |
| Publisher | MDPI |
| Volume, Issue, Pages | 12,pp.1224 |
| Number of pages | 13 |
| Responsible for | Corresponding author |
| Author and coauthor | Kunio Kawamura, Mari Ogawa, Noriko Konagaya, Yoshimi Maruoka, Jean-François Lambert, Louis M. P. Ter-Ovanessian, Jacques Vergne, Guy Hervé, Marie-Christine Maurel |
| Details | The RNA world hypothesis suggests that chemical networks consisting of functional RNA molecules could have constructed a primitive life-like system leading a first living system. We have demonstrated the importance of the environment at both high temperature and high pressure, using different types of hydrothermal flow reactor systems and high-pressure equipment. In the present study, we have attempted to develop an alternative easy-to-implement method for high-pressure mea- surements and demonstrate that the system is applicable as an efficient research tool for high-pressure experiments at pressures up to 30 MPa. We demonstrate the usefulness of the system by detecting the high-pressure influence for the self-cleavage of avocado hammerhead ribozyme (ASBVd(−):HHR) at 45–65 ◦C. A kinetic analysis of the high-pressure behavior of ASBVd(−):HHR shows that the ribozyme is active at 30 MPa and its activity is sensitive to pressures between 0.1–30 MPa. |