Educe University Faculty Information

Present specialized field
Academic background
Business career
Book and thesis
Academic conference presentation

（Last updated : 2025-09-23 08:58:06）

SAMBONGI Yoshihiro
Hiroshima shudo University The Faculty of Human Environmental Studies

Professor

■ Present specialized field

●Specialized field
Other, Other
●Keyword
●The main research themes
●Capable of joint research and research consultation content

■ Academic background

1.	1986/04～1988/03	The University of Tokyo Graduate School, Division of Agricultural Science 〔Master degree program〕 Completed
2.	1988/04～1991/03	The University of Tokyo Graduate School, Division of Agricultural Science 〔Doctoral course〕 Completed
3.	～1986/03	The University of Tokyo Faculty of Agriculture Graduated

■ Business career

1.	1992/06～1995/02	Oxford University Department of Biochemistry
2.	2018/04～2022/03	Manager(Dean)

■ Book and thesis

1.	Book	Extremophilic Enzymes Related to Energy Conversion (Collaboration) 2018/01
2.	Book	Rotational catalysis by F-type ATPase (Collaboration) 2000/01
3.	Thesis	Construction of a cyclic regular-triangle trimer of cytochrome c555 with a central hole using sortase A (Collaboration) 2025/05
4.	Thesis	Thermal tolerance of larvae of seven Chironomus species and up-regulation of heat shock protein-coding genes in Chironomus sulfurosus (Collaboration) 2025/01
5.	Thesis	Conformational rigidity of cytochrome c'-α from a thermophile is associated with slow NO binding (Collaboration) 2024/08
6.	Thesis	Isolation and characterization of bacteria from the gut of a mesopelagic copepod Cephalophanes reflugens (Copepoda: Calanoida) (Collaboration) 2024/01
7.	Thesis	Contribution of a surface salt bridge to the protein stability of deep-sea Shewanella benthica cytochrome c' (Collaboration) 2023/01
8.	Thesis	Lentilactobacillus hilgardii H-50 strongly inhibits lipopolysaccharide-induced inflammatory responses in mouse splenocytes via its specific surface layer proteins (Collaboration) 2023/01
9.	Thesis	Rapid and collective determination of the complete “hot-spring frog” mitochondrial genome containing long repeat regions (Collaboration) 2023/01
10.	Thesis	Species-specific microorganisms in acid-tolerant Chironomus larvae reared in a neutral pH range under laboratory conditions: Single dataset analysis (Collaboration) 2023/01
11.	Thesis	Crystal structure of thermally stable homodimeric cytochrome c'-α from Thermus thermophilus (Collaboration) 2022/01
12.	Thesis	Heterologous expression and biochemical comparison of two homologous SoxX proteins of endosymbiontic Candidatus Vesicomyosocius okutanii and free-living Hydrogenovibrio crunogenus from deep-sea environments (Collaboration) 2022/01
13.	Thesis	Fermented date residue extract mix containing gamma-aminobutyric acid augments the immune function of mouse splenocytes (Collaboration) 2021/01
14.	Thesis	Function of the TRY C-terminal region artificially fused with its homologous transcription factors inducing root hair differentiation in Arabidopsis (Collaboration) 2021/01
15.	Thesis	Identification of six CPC-like genes and their differential expression in leaves of tea plant, Camellia sinensis (Collaboration) 2021/01
16.	Thesis	Thermal destabilization mechanism of cytochrome c' from psychrophilic Shewanella violacea (Collaboration) 2021/01
17.	Thesis	Thermal stability tuning without affecting gas-binding function of Thermochromatium tepidum cytochrome c' (Collaboration) 2021/01
18.	Thesis	Transcriptome analysis of Chironomus sulfurosus larvae living in acidic environments: Insights into molecular mechanisms for acid tolerance (Collaboration) 2021/01
19.	Thesis	Unexpectedly high thermostability of an NADP-dependent malic enzyme from a psychrophilic bacterium, Shewanella livingstonensis Ac10 (Collaboration) 2021/01
20.	Thesis	Expression of two glutamate decarboxylase genes in Lactobacillus brevis (Collaboration) 2020/01
21.	Thesis	Conferment of CO-Controlled DimerMonomer Transition Property to Thermostable Cytochrome c' by Mutation in the SubunitSubunit Interface (Collaboration) 2019/01
22.	Thesis	Differences in biochemical properties of two 5′-nucleotidases from deep- and shallow-sea Shewanella species under various harsh conditions (Collaboration) 2019/01
23.	Thesis	One fold, two functions: cytochrome P460 and cytochrome c'-α from the methanotroph Methylococcus capsulatus (Bath) (Collaboration) 2019/01
24.	Thesis	Response of neutrophilic Shewanella violacea to acid stress: Growth rate, organic acid production, and gene expression (Collaboration) 2019/01
25.	Thesis	Stability of cytochromes c' from psychrophilic and piezophilic Shewanella species: Implications for complex multiple adaptation to low temperature and high hydrostatic pressure (Collaboration) 2019/01
26.	Thesis	Commonly stabilized cytochromes c from deep-sea Shewanella and Pseudomonas (Collaboration) 2018/01
27.	Thesis	Gamma-aminobutyric acid fermentation with date residue by a lactic acid bacterium, Lactobacillus brevis (Collaboration) 2018/01
28.	Thesis	Stabilization of mesophilic Allochromatium vinosum cytochrome c′ through specific mutations modeled by a thermophilic homologue (Collaboration) 2018/01
29.	Thesis	Difference in NaCl tolerance of membrane-bound 5′-nucleotidases purified from deep-sea and brackish water Shewanella species (Collaboration) 2017/01
30.	Thesis	Pseudomonas aeruginosa cytochrome c551 denaturation by five systematic urea derivatives that differ in the alkyl chain length (Collaboration) 2017/01
31.	Thesis	Pyrophosphate hydrolysis in the extremely halophilic archaeon Haloarcula japonica is catalyzed by a single enzyme with a broad ionic strength range (Collaboration) 2017/01
32.	Thesis	Structural and functional insights into thermally stable cytochrome c' from a thermophile (Collaboration) 2017/01
33.	Thesis	Comparative study on stabilization mechanism of monomeric cytochrome c5 from deep-sea piezophilic Shewanella violacea (Collaboration) 2016/01
34.	Thesis	Thermal stability of cytochrome c' from mesophilic Shewanella amazonensis (Collaboration) 2015/01
35.	Thesis	Correlation between the optimal growth pressures of four Shewanella species and the stabilities of their cytochromes c5 (Collaboration) 2014/01
36.	Thesis	High stability of apo-cytochrome c´ from thermophilic Hydrogenophilus thermoluteolus (Collaboration) 2014/01
37.	Thesis	Constant enthalpy change value during pyrophosphate hydrolysis within the physiological limits of NaCl (Collaboration) 2013/01
38.	Thesis	High thermal stability and unique trimer formation of cytochrome c´　from thermophilic Hydrogenophilus thermoluteolus (Collaboration) 2013/01
39.	Thesis	Hydration state change of horse heart cytochrome c corresponding to trifluoroacetic-acid-induced unfolding (Collaboration) 2013/01
40.	Thesis	Oxidative phosphorylation in a thermophilic, facultative chemoautotroph, Hydrogenophilus thermoluteolus, living prevalently in geothermal niches (Collaboration) 2013/01
41.	Thesis	Regulation of cytochrome c- and quinol oxidases and piezotolerance of the activities in the deep-sea piezophile Shewanella violacea DSS12 in response to growth conditions (Collaboration) 2013/01
42.	Thesis	Long-term observation of fluorescence of free single molecules to explore protein-folding energy landscapes (Collaboration) 2012/01
43.	Thesis	Transcriptome analyses for metabolic enzymes in thiosulfate- and hydrogen-grown Hydrogenobacter thermophilus cells (Collaboration) 2012/01
44.	Thesis	Conferment of folding ability to a naturally unfolded apocytochrome c through introduction of hydrophobic amino acid residues (Collaboration) 2011/01
45.	Thesis	Effects of heme on the thermal stability of mesophilic and thermophilic cytochromes c: Comparison between experimental and theoretical results (Collaboration) 2011/01
46.	Thesis	Heterologous synthesis of cytochrome c´ by Escherichia coli is not dependent on the System I cytochrome c biogenesis machinery (Collaboration) 2011/01
47.	Thesis	Hydration analysis of Pseudomonas aeruginosa cytochrome c551 upon acid unfolding by dielectric relaxation spectroscopy (Collaboration) 2011/01
48.	Thesis	Piezotolerance of the respiratory terminal oxidase activity of the piezophilic Shewanella violacea DSS12 compared with non-piezophilic Shewanella species (Collaboration) 2011/01
49.	Thesis	Thermal stability of cytochromes c5 from pressure-sensitive Shewanella livingstonensis (Collaboration) 2011/01
50.	Thesis	Comparative analysis of highly homologous cytochromes c5 for stability and function (Collaboration) 2010/01
51.	Thesis	Thiosulfate oxidation by a thermo-neutrophic hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus (Collaboration) 2010/01
52.	Thesis	Correlation between stability and redox potential of three homologous cytochromes c from two thermophiles and one mesophile (Collaboration) 2009/01
53.	Thesis	Effects of cysteine introduction into three homologous cytochromes c (Collaboration) 2009/01
54.	Thesis	Heme is not required for Aquifex aeolicus cytochrome c555 polypeptide folding (Collaboration) 2009/01
55.	Thesis	Hyperstability and crystal structure of cytochrome c555 from hyperthermophilic Aquifex aeolicus (Collaboration) 2009/01
56.	Thesis	Stability enhancement of cytochrome c through heme deprotonation and mutations (Collaboration) 2009/01
57.	Thesis	Stabilization mechanism of cytochrome c552 from a moderately thermophilic bacterium, Hydrogenophilus thermoluteolus (Collaboration) 2008/01
58.	Thesis	Roles of a short connecting disulfide bond in the stability and function of psychrophilic Shewanella violacea cytochrome c5 (Collaboration) 2007/01
59.	Thesis	Thiosulfate oxidation by a moderately thermophilic hydrogen-oxidizing bacterium, Hydrogenophilus thermoluteolus (Collaboration) 2007/01
60.	Thesis	Further enhancement of the thermostability of Hydrogenobacter thermophilus cytochrome c552 (Collaboration) 2006/01
61.	Thesis	Influence of a single amide group on the redox function of Pseudomonas aeruginosa ferricytochrome c551 (Collaboration) 2006/01
62.	Thesis	Structure of cytochrome c552 from a moderate thermophilic bacterium, Hydrogenophilus thermoluteolus: Comparative study on the thermostability of cytochrome c (Collaboration) 2006/01
63.	Thesis	Characterization of non-native heme coordination structures emerging upon guanidine hydrochloric acid-induced unfolding of Pseudomonas aeruginosa ferricytochrome c551 (Collaboration) 2005/01
64.	Thesis	Cloning, expression, crystallization, and preliminary X-ray characterization of cytochrome c552 from a moderate thermophilic bacterium, Hydrogenophilus thermoluteolus (Collaboration) 2005/01
65.	Thesis	Control of the redox potential of Pseudomonas aeruginosa cytochrome c551 through the Fe-Met coordination bond strength and pKa of a buried heme propionic acid side chain (Collaboration) 2005/01
66.	Thesis	Five amino acid residues responsible for the high stability of Hydrogenobacter thermophilus cytochrome c552: RECIPROCAL MUTATION ANALYSIS (Collaboration) 2005/01
67.	Thesis	Unexpected and elevated production of Aquifex aeolicus cytochrome c555 in Escherichia coli cells lacking disulfide oxidoreductases (Collaboration) 2005/01
68.	Thesis	Why isn't 'standard' heme good enough for c-type and d1-type cytochromes? (Collaboration) 2005/01
69.	Thesis	Complete thermal-unfolding profiles of oxidized and reduced cytochromes c (Collaboration) 2004/01
70.	Thesis	Effects of axial methionine coordination on the in-plane asymmetry of the heme electronic structure of cytochrome c (Collaboration) 2004/01
71.	Thesis	Relationship between redox function and protein stability of cytochromes c (Collaboration) 2003/01
72.	Thesis	A novel water-soluble Hantzsch 1, 4-dihydropyridine compound that functions in biological processes through NADH regeneration (Collaboration) 2002/01
73.	Thesis	Cytochrome c from a thermophilic bacterium has provided insights into the mechanism of protein maturation, folding, and stability (Collaboration) 2002/01
74.	Thesis	Influence of amino acid side-chain packing on Fe-methionine coordination in thermostable cytochrome c (Collaboration) 2002/01
75.	Thesis	Rotation of a complex of the β subunit and c ring of Escherichia coli ATP synthase: The rotor and stator are interchangeable (Collaboration) 2002/01
76.	Thesis	Thermodynamic characterization of mesophilic cytochrome c variants and its thermophilic counterpart (Collaboration) 2002/01
77.	Thesis	A biological molecular motor, proton translocating ATP synthase: Multidisciplinary approach to a unique membrane enzyme (Collaboration) 2001/01
78.	Thesis	ATP synthase F1 sector rotation. Defective torque generation in the β subunit Ser-174 to Phe mutant and its suppression by second mutations (Collaboration) 2001/01
79.	Thesis	Biological nano motor FoF1 ATP synthase: Catalysis leading to c subunit oligomer rotation (Collaboration) 2001/01
80.	Thesis	Synthase (H+ ATPase): Coupling between catalysis, mechanical work, and proton translocation (Collaboration) 2001/01
81.	Thesis	Caenorhabditis elegans senses protons through amphid chemosensory neurons: Proton signals elicit avoidance behavior (Collaboration) 2000/01
82.	Thesis	Evidence that single missense mutations in the Atp7a gene of two mouse models for Menkes’s disease are responsible for impared copper transport (Collaboration) 2000/01
83.	Thesis	Selected mutations in a mesophilic cytochrome c confer the stability of a thermophilic counterpart (Collaboration) 2000/01
84.	Thesis	Mechanical rotation of c subunit oligomer in ATP synthase (FoF1): Direct observation (Collaboration) 1999/01
85.	Thesis	Sensing of cadmium and copper ions by externally exposed ADL, ASE, and ASH neurons elicits avoidance response in Caenorhabditis elegans (Collaboration) 1999/01
86.	Thesis	Stabilization of Pseudomonas aeruginosa cytochrome c-551 by systematic amino acid substitutions based on the structure of thermophilic Hydrogenobacter thermophilus cytochrome c-552 (Collaboration) 1999/01
87.	Thesis	The γ-subunit rotation and torque generation in F1-ATPase from wild-type or uncoupled mutant Escherichia coli (Collaboration) 1999/01
88.	Thesis	Analysis of functional domains of Wilson disease protein (ATP7B) in Saccharomyces cerevisiae (Collaboration) 1998/01
89.	Thesis	Contrasting routes of c-type cytochrome assembly in mitochondria, chloroplast and bacteria (Collaboration) 1998/01
90.	Thesis	Essential Cys-Pro-Cys motif of Caenorhabditis elegans copper transport ATPase (Collaboration) 1998/01
91.	Thesis	Heterologous expression of Hydrogenobacter thermophilus cytochrome c-552 in the periplasm of Pseudomonas aeruginosa (Collaboration) 1998/01
92.	Thesis	Identification of the copper chaperone, CUC-1, in Caenorhabditis elegans: tissue specific co-expression with the copper transporting ATPase, CUA-1 (Collaboration) 1998/01
93.	Thesis	Solution structure of a thermostable cytochrome c-552 from Hydrogenobacter thermophilus determined by 1H-NMR (Collaboration) 1998/01
94.	Thesis	Caenorhabditis elegans cDNA for Menkes/Wilson disease gene homologue and its function in yeast CCC2 gene disruption mutant (Collaboration) 1997/01
95.	Thesis	Alteration of haem-attachment and signal-cleavage sites for Paracoccus denitrificans cytochrome c550 probes pathway of c-type cytochrome biogenesis in Escherichia coli (Collaboration) 1996/01
96.	Thesis	Cytochrome c550 expression in Paracoccus denitrificans strongly depends on growth condition: identification of promoter region for cycA by transcriptional start analysis (Collaboration) 1996/01
97.	Thesis	Mutants of Escherichia coli lacking disulphide oxidoreductases DsbA and DsbB cannot synthesise an exogenous monohaem c-type cytochrome except in the presence of disulphide compounds (Collaboration) 1996/01
98.	Thesis	Production of recombinant cytochrome c-551 in a Pseudomonas aeruginosa mutant strain (Collaboration) 1995/01
99.	Thesis	A mutation blocking the formation of membrane or periplasmic endogenous and exogenous c-type cytochromes in Escherichia coli permits the cytoplasmic formation of Hydrogenobacter thermophilus holo cytochrome c552 (Collaboration) 1994/01
100.	Thesis	Specific thiol compounds complement deficiency in c-type cytochrome biogenesis in Escherichia coli carrying a mutation in a membrane-bound disulphide isomerase-like protein (Collaboration) 1994/01
101.	Thesis	Synthesis of holo Paracoccus denitrificans cytochrome c550 requires targeting to the periplasm whereas that of holo Hydrogenobacter thermophilus cytochrome c552 does not. Implication for c-type cytochrome biogenesis (Collaboration) 1994/01
102.	Thesis	Regulation and sequence of the structural gene for cytochrome c552 from Escherichia coli: not a hexahaem but a 50 kDa tetrahaem nitrite reductase (Collaboration) 1993/01
103.	Thesis	Thermostability of Pseudomonas hydrogenothermophila cytochrome c-552 (Collaboration) 1992/01
104.	Thesis	Cloning, nucleotide sequence, and expression of the cytochrome c-552 gene from Hydrogenobacter thermophilus (Collaboration) 1991/01
105.	Thesis	Crystallization and preliminary X-ray diffraction study of cytochrome c552 from Hydrogenobacter thermophilus (Collaboration) 1991/01
106.	Thesis	Cloning and sequencing of the gene encoding cytochrome c-551 from Pseudomonas aeruginosa (Collaboration) 1990/01
107.	Thesis	Amino acid sequence of cytochrome c-552 from a thermophilic hydrogen-oxidizing bacterium Hydrogenobacter thermophilus (Collaboration) 1989/01
108.	Thesis	Thermostability of cytochrome c-552 from a thermophilic hydrogen-oxidizing bacterium Hydrogenobacter thermophilus (Collaboration) 1989/01
109.	Thesis	The drug resistance of Hydrogenobacter thermophilus strain TK-6 (Collaboration) 1988/01
5display
All display(109)

■ Academic conference presentation

1.	2024/06/02	Chitinoclastic activities of bacteria isolated from the gut of a deep-sea copepod Cephalophanes reflugens (Copepoda: Calanoida) (15TH INTERNATIONAL CONFERENCE ON COPEPODA)
2.	2019/09/02	Thermal stability of cytochrome c from thermophilic Thermus thermophilus (International Congress on Thermophiles)
3.	2019/06/02	Conferment of CO-Controlled Dimer–Monomer Transition Property to Thermostable Cytochrome c by Mutation in the Subunit Interface (15th International Symposium on Applied Bioinorganic Chemistry)
4.	2019/06/01	Isovaleric acid production as the response to acid stress in Shewanella violacea (日本農芸化学会中四国支部会第54回講演会)
5.	2018/09/10	Microbial corrosion induced by co-existence of Acetobacterium sp. and Desulfovibrio sp. enriched from bottom water in oil-storage tank (Eurocorr2018)
6.	2018/03/09	GABA production with date residue (Triple Helix International Seminar: Linking University-Industry-Government)
7.	2017/06/17	Shewanella violacea genes expressed under acidic and neutral conditions (農芸化学中四国支部第48回講演会)
8.	2017/06/03	Metallic iron corrosion by microorganisms living in oil-storage tanks (ASM Microbe 2017)
9.	2016/09/12	Functional and Structural Molecular Adaptation Strategy in Cytochrome c' from Thermophilic Hydrogenophilus thermoluteolus (the 11th International Congress on Extremophiles)
10.	2016/09/11	Difference in salt stability of membrane-bound 5’-nucleotidases purified from piezophilic, moderately-halophilic and piezosensitive, non-halophilic Shewanella species (5th International Workshop on Deep sea Microbiology)
11.	2016/09/10	Comparative study on stabilization mechanism of monomeric cytochrome c5 from deep-sea piezophilic Shewanella violacea (5th International Workshop on Deep sea Microbiology)
12.	2015/09/15	Proteins designed by extremophiles (第53回日本生物物理学会年会)
13.	2014/09/10	Correlation between the optimal growth pressures of four Shewanella species and the stabilities of their cytochromes c (1st SPIRITS Workshop)
14.	2014/09/10	Thermal stability and stabilization mechanism of cytochrome c' from thermophilic Hydrogenophilus thermoluteolus (10th International Congress on Extremophiles)
15.	2014/07/08	EVALUATION OF DIAGNOSTIC METHODS FOR MICROBIOLOGICALLY INFLUENCED CORROSION (17th International Congress on Marine Corrosion and Fouling)
16.	2001/03/01	Mechanical coupling of FoF1 (The 4th Sanken international symposium, (Osaka Japan))
17.	1998/07/05	Physiological roles of C. elegans copper transport ATPase. (European Worm Meeting,)
18.	1994/09/16	C-type cytochrome biogenesis in Escherichia coli (8th European Bioenergetics Conference)
5display
All display(18)