Educe University Faculty Information

Present specialized field
Academic background
Business career
Book and thesis
Academic conference presentation
Subject

（Last updated : 2025-07-16 10:31:57）

SAMBONGI Yoshihiro
Hiroshima shudo University The Faculty of Human Environmental Studies

Professor

■ Present specialized field

●Specialized field
Other, Other
●Keyword
●The main research themes
●Capable of joint research and research consultation content

■ Academic background

1.	1986/04～1988/03	The University of Tokyo Graduate School, Division of Agricultural Science 〔Master degree program〕 Completed
2.	1988/04～1991/03	The University of Tokyo Graduate School, Division of Agricultural Science 〔Doctoral course〕 Completed
3.	～1986/03	The University of Tokyo Faculty of Agriculture Graduated

■ Business career

1992/06～1995/02

Oxford University Department of Biochemistry

■ Book and thesis

1.	Book	Extremophilic Enzymes Related to Energy Conversion (Collaboration) 2018/01
2.	Book	Rotational catalysis by F-type ATPase (Collaboration) 2000/01
3.	Thesis	Thermal tolerance of larvae of seven Chironomus species and up-regulation of heat shock protein-coding genes in Chironomus sulfurosus (Collaboration) 2025/01
4.	Thesis	Conformational rigidity of cytochrome c'-α from a thermophile is associated with slow NO binding (Collaboration) 2024/08
5.	Thesis	Conformational rigidity of cytochrome c'-α from a thermophile is associated with slow NO binding (Collaboration) 2024/01
6.	Thesis	Isolation and characterization of bacteria from the gut of a mesopelagic copepod Cephalophanes reflugens (Copepoda: Calanoida) (Collaboration) 2024/01
7.	Thesis	Contribution of a surface salt bridge to the protein stability of deep-sea Shewanella benthica cytochrome c' (Collaboration) 2023/01
8.	Thesis	Lentilactobacillus hilgardii H-50 strongly inhibits lipopolysaccharide-induced inflammatory responses in mouse splenocytes via its specific surface layer proteins (Collaboration) 2023/01
9.	Thesis	Rapid and collective determination of the complete “hot-spring frog” mitochondrial genome containing long repeat regions (Collaboration) 2023/01
10.	Thesis	Species-specific microorganisms in acid-tolerant Chironomus larvae reared in a neutral pH range under laboratory conditions: Single dataset analysis (Collaboration) 2023/01
11.	Thesis	Crystal structure of thermally stable homodimeric cytochrome c'- from Thermus thermophilus (Collaboration) 2022/01
12.	Thesis	Heterologous expression and biochemical comparison of two homologous SoxX proteins of endosymbiontic Candidatus Vesicomyosocius okutanii and free-living Hydrogenovibrio crunogenus from deep-sea environments (Collaboration) 2022/01
13.	Thesis	Fermented date residue extract mix containing gamma-aminobutyric acid augments the immune function of mouse splenocytes (Collaboration) 2021/01
14.	Thesis	Function of the TRY C-terminal region artificially fused with its homologous transcription factors inducing root hair differentiation in Arabidopsis (Collaboration) 2021/01
15.	Thesis	Identification of six CPC-like genes and their differential expression in leaves of tea plant, Camellia sinensis (Collaboration) 2021/01
16.	Thesis	Thermal destabilization mechanism of cytochrome c' from psychrophilic Shewanella violacea (Collaboration) 2021/01
17.	Thesis	Thermal stability tuning without affecting gas-binding function of Thermochromatium tepidum cytochrome c' (Collaboration) 2021/01
18.	Thesis	Transcriptome analysis of Chironomus sulfurosus larvae living in acidic environments: Insights into molecular mechanisms for acid tolerance (Collaboration) 2021/01
19.	Thesis	Unexpectedly high thermostability of an NADP-dependent malic enzyme from a psychrophilic bacterium, Shewanella livingstonensis Ac10 (Collaboration) 2021/01
20.	Thesis	Expression of two glutamate decarboxylase genes in Lactobacillus brevis (Collaboration) 2020/01
21.	Thesis	Conferment of CO-Controlled DimerMonomer Transition Property to Thermostable Cytochrome c' by Mutation in the SubunitSubunit Interface (Collaboration) 2019/01
22.	Thesis	Differences in biochemical properties of two 5′-nucleotidases from deep- and shallow-sea Shewanella species under various harsh conditions (Collaboration) 2019/01
23.	Thesis	One fold, two functions: cytochrome P460 and cytochrome c'- from the methanotroph Methylococcus capsulatus (Bath) (Collaboration) 2019/01
24.	Thesis	Response of neutrophilic Shewanella violacea to acid stress: Growth rate, organic acid production, and gene expression (Collaboration) 2019/01
25.	Thesis	Stability of cytochromes c' from psychrophilic and piezophilic Shewanella species: Implications for complex multiple adaptation to low temperature and high hydrostatic pressure (Collaboration) 2019/01
26.	Thesis	Commonly stabilized cytochromes c from deep-sea Shewanella and Pseudomonas (Collaboration) 2018/01
27.	Thesis	Gamma-aminobutyric acid fermentation with date residue by a lactic acid bacterium, Lactobacillus brevis (Collaboration) 2018/01
28.	Thesis	Stabilization of mesophilic Allochromatium vinosum cytochrome c′ through specific mutations modeled by a thermophilic homologue (Collaboration) 2018/01
29.	Thesis	Difference in NaCl tolerance of membrane-bound 5′-nucleotidases purified from deep-sea and brackish water Shewanella species (Collaboration) 2017/01
30.	Thesis	Pseudomonas aeruginosa cytochrome c551 denaturation by five systematic urea derivatives that differ in the alkyl chain length (Collaboration) 2017/01
31.	Thesis	Pyrophosphate hydrolysis in the extremely halophilic archaeon Haloarcula japonica is catalyzed by a single enzyme with a broad ionic strength range (Collaboration) 2017/01
32.	Thesis	Structural and functional insights into thermally stable cytochrome c' from a thermophile (Collaboration) 2017/01
33.	Thesis	Comparative study on stabilization mechanism of monomeric cytochrome c5 from deep-sea piezophilic Shewanella violacea (Collaboration) 2016/01
34.	Thesis	Thermal stability of cytochrome c' from mesophilic Shewanella amazonensis (Collaboration) 2015/01
35.	Thesis	Correlation between the optimal growth pressures of four Shewanella species and the stabilities of their cytochromes c5 (Collaboration) 2014/01
36.	Thesis	High stability of apo-cytochrome c´ from thermophilic Hydrogenophilus thermoluteolus (Collaboration) 2014/01
37.	Thesis	Constant enthalpy change value during pyrophosphate hydrolysis within the physiological limits of NaCl (Collaboration) 2013/01
38.	Thesis	High thermal stability and unique trimer formation of cytochrome c´　from thermophilic Hydrogenophilus thermoluteolus (Collaboration) 2013/01
39.	Thesis	Hydration state change of horse heart cytochrome c corresponding to trifluoroacetic-acid-induced unfolding (Collaboration) 2013/01
40.	Thesis	Oxidative phosphorylation in a thermophilic, facultative chemoautotroph, Hydrogenophilus thermoluteolus, living prevalently in geothermal niches (Collaboration) 2013/01
41.	Thesis	Regulation of cytochrome c- and quinol oxidases and piezotolerance of the activities in the deep-sea piezophile Shewanella violacea DSS12 in response to growth conditions (Collaboration) 2013/01
42.	Thesis	Long-term observation of fluorescence of free single molecules to explore protein-folding energy landscapes (Collaboration) 2012/01
43.	Thesis	Transcriptome analyses for metabolic enzymes in thiosulfate- and hydrogen-grown Hydrogenobacter thermophilus cells (Collaboration) 2012/01
44.	Thesis	Conferment of folding ability to a naturally unfolded apocytochrome c through introduction of hydrophobic amino acid residues (Collaboration) 2011/01
45.	Thesis	Effects of heme on the thermal stability of mesophilic and thermophilic cytochromes c: Comparison between experimental and theoretical results (Collaboration) 2011/01
46.	Thesis	Heterologous synthesis of cytochrome c´ by Escherichia coli is not dependent on the System I cytochrome c biogenesis machinery (Collaboration) 2011/01
47.	Thesis	Hydration analysis of Pseudomonas aeruginosa cytochrome c551 upon acid unfolding by dielectric relaxation spectroscopy (Collaboration) 2011/01
48.	Thesis	Piezotolerance of the respiratory terminal oxidase activity of the piezophilic Shewanella violacea DSS12 compared with non-piezophilic Shewanella species (Collaboration) 2011/01
49.	Thesis	Thermal stability of cytochromes c5 from pressure-sensitive Shewanella livingstonensis (Collaboration) 2011/01
50.	Thesis	Comparative analysis of highly homologous cytochromes c5 for stability and function (Collaboration) 2010/01
51.	Thesis	Thiosulfate oxidation by a thermo-neutrophic hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus (Collaboration) 2010/01
52.	Thesis	Correlation between stability and redox potential of three homologous cytochromes c from two thermophiles and one mesophile (Collaboration) 2009/01
53.	Thesis	Effects of cysteine introduction into three homologous cytochromes c (Collaboration) 2009/01
54.	Thesis	Heme is not required for Aquifex aeolicus cytochrome c555 polypeptide folding (Collaboration) 2009/01
55.	Thesis	Hyperstability and crystal structure of cytochrome c555 from hyperthermophilic Aquifex aeolicus (Collaboration) 2009/01
56.	Thesis	Stability enhancement of cytochrome c through heme deprotonation and mutations (Collaboration) 2009/01
57.	Thesis	Stabilization mechanism of cytochrome c552 from a moderately thermophilic bacterium, Hydrogenophilus thermoluteolus (Collaboration) 2008/01
58.	Thesis	Roles of a short connecting disulfide bond in the stability and function of psychrophilic Shewanella violacea cytochrome c5 (Collaboration) 2007/01
59.	Thesis	Thiosulfate oxidation by a moderately thermophilic hydrogen-oxidizing bacterium, Hydrogenophilus thermoluteolus (Collaboration) 2007/01
60.	Thesis	Further enhancement of the thermostability of Hydrogenobacter thermophilus cytochrome c552 (Collaboration) 2006/01
61.	Thesis	Influence of a single amide group on the redox function of Pseudomonas aeruginosa ferricytochrome c551 (Collaboration) 2006/01
62.	Thesis	Structure of cytochrome c552 from a moderate thermophilic bacterium, Hydrogenophilus thermoluteolus: Comparative study on the thermostability of cytochrome c (Collaboration) 2006/01
63.	Thesis	Characterization of non-native heme coordination structures emerging upon guanidine hydrochloric acid-induced unfolding of Pseudomonas aeruginosa ferricytochrome c551 (Collaboration) 2005/01
64.	Thesis	Cloning, expression, crystallization, and preliminary X-ray characterization of cytochrome c552 from a moderate thermophilic bacterium, Hydrogenophilus thermoluteolus (Collaboration) 2005/01
65.	Thesis	Control of the redox potential of Pseudomonas aeruginosa cytochrome c551 through the Fe-Met coordination bond strength and pKa of a buried heme propionic acid side chain (Collaboration) 2005/01
66.	Thesis	Five amino acid residues responsible for the high stability of Hydrogenobacter thermophilus cytochrome c552: RECIPROCAL MUTATION ANALYSIS (Collaboration) 2005/01
67.	Thesis	Unexpected and elevated production of Aquifex aeolicus cytochrome c555 in Escherichia coli cells lacking disulfide oxidoreductases (Collaboration) 2005/01
68.	Thesis	Why isn't 'standard' heme good enough for c-type and d1-type cytochromes? (Collaboration) 2005/01
69.	Thesis	Complete thermal-unfolding profiles of oxidized and reduced cytochromes c (Collaboration) 2004/01
70.	Thesis	Effects of axial methionine coordination on the in-plane asymmetry of the heme electronic structure of cytochrome c (Collaboration) 2004/01
71.	Thesis	Relationship between redox function and protein stability of cytochromes c (Collaboration) 2003/01
72.	Thesis	A novel water-soluble Hantzsch 1, 4-dihydropyridine compound that functions in biological processes through NADH regeneration (Collaboration) 2002/01
73.	Thesis	Cytochrome c from a thermophilic bacterium has provided insights into the mechanism of protein maturation, folding, and stability (Collaboration) 2002/01
74.	Thesis	Influence of amino acid side-chain packing on Fe-methionine coordination in thermostable cytochrome c (Collaboration) 2002/01
75.	Thesis	Rotation of a complex of the  subunit and c ring of Escherichia coli ATP synthase: The rotor and stator are interchangeable (Collaboration) 2002/01
76.	Thesis	Thermodynamic characterization of mesophilic cytochrome c variants and its thermophilic counterpart (Collaboration) 2002/01
77.	Thesis	A biological molecular motor, proton translocating ATP synthase: Multidisciplinary approach to a unique membrane enzyme (Collaboration) 2001/01
78.	Thesis	ATP synthase F1 sector rotation. Defective torque generation in the b subunit Ser-174 to Phe mutant and its suppression by second mutations (Collaboration) 2001/01
79.	Thesis	Biological nano motor FoF1 ATP synthase: Catalysis leading to c subunit oligomer rotation (Collaboration) 2001/01
80.	Thesis	Synthase (H+ ATPase): Coupling between catalysis, mechanical work, and proton translocation (Collaboration) 2001/01
81.	Thesis	Caenorhabditis elegans senses protons through amphid chemosensory neurons: Proton signals elicit avoidance behavior (Collaboration) 2000/01
82.	Thesis	Evidence that single missense mutations in the Atp7a gene of two mouse models for Menkes’s disease are responsible for impared copper transport (Collaboration) 2000/01
83.	Thesis	Selected mutations in a mesophilic cytochrome c confer the stability of a thermophilic counterpart (Collaboration) 2000/01
84.	Thesis	Mechanical rotation of c subunit oligomer in ATP synthase (FoF1): Direct observation (Collaboration) 1999/01
85.	Thesis	Sensing of cadmium and copper ions by externally exposed ADL, ASE, and ASH neurons elicits avoidance response in Caenorhabditis elegans (Collaboration) 1999/01
86.	Thesis	Stabilization of Pseudomonas aeruginosa cytochrome c-551 by systematic amino acid substitutions based on the structure of thermophilic Hydrogenobacter thermophilus cytochrome c-552 (Collaboration) 1999/01
87.	Thesis	The g-subunit rotation and torque generation in F1-ATPase from wild-type or uncoupled mutant Escherichia coli (Collaboration) 1999/01
88.	Thesis	Analysis of functional domains of Wilson disease protein (ATP7B) in Saccharomyces cerevisiae (Collaboration) 1998/01
89.	Thesis	Contrasting routes of c-type cytochrome assembly in mitochondria, chloroplast and bacteria (Collaboration) 1998/01
90.	Thesis	Essential Cys-Pro-Cys motif of Caenorhabditis elegans copper transport ATPase (Collaboration) 1998/01
91.	Thesis	Heterologous expression of Hydrogenobacter thermophilus cytochrome c-552 in the periplasm of Pseudomonas aeruginosa (Collaboration) 1998/01
92.	Thesis	Identification of the copper chaperone, CUC-1, in Caenorhabditis elegans: tissue specific co-expression with the copper transporting ATPase, CUA-1 (Collaboration) 1998/01
93.	Thesis	Solution structure of a thermostable cytochrome c-552 from Hydrogenobacter thermophilus determined by 1H-NMR (Collaboration) 1998/01
94.	Thesis	Caenorhabditis elegans cDNA for Menkes/Wilson disease gene homologue and its function in yeast CCC2 gene disruption mutant (Collaboration) 1997/01
95.	Thesis	Alteration of haem-attachment and signal-cleavage sites for Paracoccus denitrificans cytochrome c550 probes pathway of c-type cytochrome biogenesis in Escherichia coli (Collaboration) 1996/01
96.	Thesis	Cytochrome c550 expression in Paracoccus denitrificans strongly depends on growth condition: identification of promoter region for cycA by transcriptional start analysis (Collaboration) 1996/01
97.	Thesis	Mutants of Escherichia coli lacking disulphide oxidoreductases DsbA and DsbB cannot synthesise an exogenous monohaem c-type cytochrome except in the presence of disulphide compounds (Collaboration) 1996/01
98.	Thesis	Production of recombinant cytochrome c-551 in a Pseudomonas aeruginosa mutant strain (Collaboration) 1995/01
99.	Thesis	A mutation blocking the formation of membrane or periplasmic endogenous and exogenous c-type cytochromes in Escherichia coli permits the cytoplasmic formation of Hydrogenobacter thermophilus holo cytochrome c552 (Collaboration) 1994/01
100.	Thesis	Specific thiol compounds complement deficiency in c-type cytochrome biogenesis in Escherichia coli carrying a mutation in a membrane-bound disulphide isomerase-like protein (Collaboration) 1994/01
101.	Thesis	Synthesis of holo Paracoccus denitrificans cytochrome c550 requires targeting to the periplasm whereas that of holo Hydrogenobacter thermophilus cytochrome c552 does not. Implication for c-type cytochrome biogenesis (Collaboration) 1994/01
102.	Thesis	Regulation and sequence of the structural gene for cytochrome c552 from Escherichia coli: not a hexahaem but a 50 kDa tetrahaem nitrite reductase (Collaboration) 1993/01
103.	Thesis	Thermostability of Pseudomonas hydrogenothermophila cytochrome c-552 (Collaboration) 1992/01
104.	Thesis	Cloning, nucleotide sequence, and expression of the cytochrome c-552 gene from Hydrogenobacter thermophilus (Collaboration) 1991/01
105.	Thesis	Crystallization and preliminary X-ray diffraction study of cytochrome c552 from Hydrogenobacter thermophilus (Collaboration) 1991/01
106.	Thesis	Cloning and sequencing of the gene encoding cytochrome c-551 from Pseudomonas aeruginosa (Collaboration) 1990/01
107.	Thesis	Amino acid sequence of cytochrome c-552 from a thermophilic hydrogen-oxidizing bacterium Hydrogenobacter thermophilus (Collaboration) 1989/01
108.	Thesis	Thermostability of cytochrome c-552 from a thermophilic hydrogen-oxidizing bacterium Hydrogenobacter thermophilus (Collaboration) 1989/01
109.	Thesis	The drug resistance of Hydrogenobacter thermophilus strain TK-6 (Collaboration) 1988/01
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■ Academic conference presentation

1.	2024/06/02	Chitinoclastic activities of bacteria isolated from the gut of a deep-sea copepod Cephalophanes reflugens (Copepoda: Calanoida) (15TH INTERNATIONAL CONFERENCE ON COPEPODA)
2.	2019/09/02	Thermal stability of cytochrome c from thermophilic Thermus thermophilus (International Congress on Thermophiles)
3.	2019/06/02	Conferment of CO-Controlled Dimer–Monomer Transition Property to Thermostable Cytochrome c by Mutation in the Subunit Interface (15th International Symposium on Applied Bioinorganic Chemistry)
4.	2019/06/01	Isovaleric acid production as the response to acid stress in Shewanella violacea (日本農芸化学会中四国支部会第54回講演会)
5.	2018/09/10	Microbial corrosion induced by co-existence of Acetobacterium sp. and Desulfovibrio sp. enriched from bottom water in oil-storage tank (Eurocorr2018)
6.	2018/03/09	GABA production with date residue (Triple Helix International Seminar: Linking University-Industry-Government)
7.	2017/06/17	Shewanella violacea genes expressed under acidic and neutral conditions (農芸化学中四国支部第48回講演会)
8.	2017/06/03	Metallic iron corrosion by microorganisms living in oil-storage tanks (ASM Microbe 2017)
9.	2016/09/12	Functional and Structural Molecular Adaptation Strategy in Cytochrome c' from Thermophilic Hydrogenophilus thermoluteolus (the 11th International Congress on Extremophiles)
10.	2016/09/11	Difference in salt stability of membrane-bound 5’-nucleotidases purified from piezophilic, moderately-halophilic and piezosensitive, non-halophilic Shewanella species (5th International Workshop on Deep sea Microbiology)
11.	2016/09/10	Comparative study on stabilization mechanism of monomeric cytochrome c5 from deep-sea piezophilic Shewanella violacea (5th International Workshop on Deep sea Microbiology)
12.	2015/09/15	Proteins designed by extremophiles (第53回日本生物物理学会年会)
13.	2014/09/10	Correlation between the optimal growth pressures of four Shewanella species and the stabilities of their cytochromes c (1st SPIRITS Workshop)
14.	2014/09/10	Thermal stability and stabilization mechanism of cytochrome c' from thermophilic Hydrogenophilus thermoluteolus (10th International Congress on Extremophiles)
15.	2014/07/08	EVALUATION OF DIAGNOSTIC METHODS FOR MICROBIOLOGICALLY INFLUENCED CORROSION (17th International Congress on Marine Corrosion and Fouling)
16.	2001/03/01	Mechanical coupling of FoF1 (The 4th Sanken international symposium, (Osaka Japan))
17.	1998/07/05	Physiological roles of C. elegans copper transport ATPase. (European Worm Meeting,)
18.	1994/09/16	C-type cytochrome biogenesis in Escherichia coli (8th European Bioenergetics Conference)
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■ Subject

1.	Freshperson Seminar
2.	Lecture(Business Theory for Society)
3.	Lecture(Business Theory for Society)
4.	Special Foundational Lecture (Microbial Biochemistry and Human Relations)
5.	Special Foundational Lecture (Microbial Biochemistry and Human Relations)
6.	Special Lecture for Introduction(Food Culture)
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